Western blot was performed using Anti-ALPL Polyclonal Antibody (Product # PA5-47419) and a 57 kDa band corresponding to ALPL was observed across the cell lines tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), SH-SY5Y (Lane 3) and MOLT-4 (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence Rabbit anti-Goat IgG Heavy Chain Superclonal Secondary Antibody, HRP conjugate (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).